› 1ufj

X-ray diffraction
1.6Å resolution

Crystal Structure of an Artificial Metalloprotein:Fe(III)(3,3'-Me2-salophen)/apo-A71G Myoglobin

Released:
DOI: 10.2210/pdb1ufj/pdb
PDB entry 1ufj coloured by chain, front view.
PDB entry 1ufj coloured by chain, side view.
PDB entry 1ufj coloured by chain, top view.
Source organism: Physeter catodon
Primary publication:
Crystal structures of artificial metalloproteins: tight binding of FeIII(Schiff-Base) by mutation of Ala71 to Gly in apo-myoglobin.
Ueno T, Ohashi M, Kono M, Kondo K, Suzuki A, Yamane T, Watanabe Y
Inorg Chem 43 2852-8 (2004)
PMID: 15106972

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 17.35 KDa
Source organism: Physeter catodon
Expression system: Escherichia coli
UniProt:
  • Canonical: P02185 (Residues: 1-154; Coverage: 100%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

3 bound ligands:
Ligand FE 1 x FE
Ligand PO4 6 x PO4
Ligand CZM 1 x CZM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: P212121
Unit cell:
a: 33.268Å b: 57.6Å c: 75.296Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.212
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Design of functional metalloproteins.
Lu et al. (2009)
13 more

2 mentions without citation

Light-driven catalysis with engineered enzymes and biomimetic systems.
Edwards et al. (2020)
1 more