PDBe 1uch

X-ray diffraction
1.8Å resolution

DEUBIQUITINATING ENZYME UCH-L3 (HUMAN) AT 1.8 ANGSTROM RESOLUTION

Released:

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase isozyme L3 Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 26.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15374 (Residues: 1-230; Coverage: 100%)
Gene name: UCHL3
Sequence domains: Ubiquitin carboxyl-terminal hydrolase, family 1
Structure domains: Ubiquitin C-terminal Hydrolase Uch-l3

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 48.6Å b: 60.9Å c: 81.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.294
Expression system: Escherichia coli