› 1uam

X-ray diffraction
2.2Å resolution

Crystal structure of tRNA(m1G37)methyltransferase: Insight into tRNA recognition

Released:
DOI: 10.2210/pdb1uam/pdb
PDB entry 1uam coloured by chain, front view.
PDB entry 1uam coloured by chain, side view.
PDB entry 1uam coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Crystal structure of tRNA(m1G37)methyltransferase: insights into tRNA recognition.
Ahn HJ, Kim HW, Yoon HJ, Lee BI, Suh SW, Yang JK
EMBO J 22 2593-603 (2003)
PMID: 12773376

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (guanine-N(1)-)-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 30.82 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P43912 (Residues: 1-246; Coverage: 100%)
Gene names: HI_0202, trmD
Sequence domains: tRNA (Guanine-1)-methyltransferase
Structure domains:

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: R32
Unit cell:
a: 92.9Å b: 92.9Å c: 179.971Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.22 0.264
Expression system: Escherichia coli

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Citations

18 review citations

7-Methylguanosine Modifications in Transfer RNA (tRNA).
Tomikawa C. (2018)
17 more

5 mentions without citation

Crystal structure of tRNA(m1G37)methyltransferase: insights into tRNA recognition.
Ahn et al. (2003)
4 more