PDBe 1u9q

X-ray diffraction
2.3Å resolution

Crystal structure of cruzain bound to an alpha-ketoester

Released:

Function and Biology Details

Reaction catalysed:
Broad endopeptidase specificity similar to that of cathepsin L. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cruzipain Chain: X
Molecule details ›
Chain: X
Length: 215 amino acids
Theoretical weight: 22.72 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: P25779 (Residues: 123-337; Coverage: 48%)
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 45.72Å b: 50.675Å c: 45.576Å
α: 90° β: 116.74° γ: 90°
R-values:
R R work R free
0.136 0.132 0.218
Expression system: Escherichia coli