› 1u88

X-ray diffraction
3.5Å resolution

Crystal Structure Of The 26 Kda Glutathione S-Transferase Y7F mutant From Schistosoma Japonicum Complexed With S-Octyl Glutathione

Released:
DOI: 10.2210/pdb1u88/pdb
PDB entry 1u88 coloured by chain, front view.
PDB entry 1u88 coloured by chain, side view.
PDB entry 1u88 coloured by chain, top view.
Source organism: Schistosoma japonicum
Primary publication:
Crystallographic and thermodynamic analysis of the binding of S-octylglutathione to the Tyr 7 to Phe mutant of glutathione S-transferase from Schistosoma japonicum.
Andújar-Sánchez M, Smith AW, Clemente-Jimenez JM, Rodriguez-Vico F, Las Heras-Vazquez FJ, Jara-Pérez V, Cámara-Artigas A
Biochemistry 44 1174-83 (2005)
PMID: 15667211

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140160 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase class-mu 26 kDa isozyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 218 amino acids
Theoretical weight: 25.52 KDa
Source organism: Schistosoma japonicum
Expression system: Escherichia coli
UniProt:
  • Canonical: P08515 (Residues: 1-218; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GTY 1 x GTY
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200HB
Spacegroup: P63
Unit cell:
a: 116.1Å b: 116.1Å c: 73.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.22 0.317
Expression system: Escherichia coli

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Citations

1 review citation

Survey of the year 2005: literature on applications of isothermal titration calorimetry.
Ababou et al. (2007)
12 other articles