› 1u7c

X-ray diffraction
1.85Å resolution

Crystal Structure of AmtB from E.Coli with Methyl Ammonium.

Released:
DOI: 10.2210/pdb1u7c/pdb
PDB entry 1u7c coloured by chain, front view.
PDB entry 1u7c coloured by chain, side view.
PDB entry 1u7c coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A.
Khademi S, O'Connell J, Remis J, Robles-Colmenares Y, Miercke LJ, Stroud RM
Science 305 1587-94 (2004)
PMID: 15361618

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159623 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ammonium transporter AmtB Chain: A
Molecule details ›
Chain: A
Length: 385 amino acids
Theoretical weight: 40.43 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69681 (Residues: 23-407; Coverage: 95%)
Gene names: JW0441, amtB, b0451, ybaG
Sequence domains: Ammonium Transporter Family
Structure domains: Ammonium transporter AmtB like domains

Ligands and Environments

1 bound ligand:
Ligand NME 1 x NME
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P63
Unit cell:
a: 95.7Å b: 95.7Å c: 94.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.195 0.205
Expression system: Escherichia coli

Quick links

Citations

97 review citations

Plant nitrogen assimilation and use efficiency.
Xu et al. (2012)
96 more

4 mentions without citation

Crystal structure of the archaeal ammonium transporter Amt-1 from Archaeoglobus fulgidus.
Andrade et al. (2005)
3 more