PDBe 1u5s

Solution NMR

NMR structure of the complex between Nck-2 SH3 domain and PINCH-1 LIM4 domain

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
LIM and senescent cell antigen-like-containing domain protein 1 Chain: B
Molecule details ›
Chain: B
Length: 66 amino acids
Theoretical weight: 7.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P48059 (Residues: 188-251; Coverage: 20%)
Gene names: LIMS1, PINCH, PINCH1
Structure domains: Cysteine Rich Protein
Cytoplasmic protein NCK2 Chain: A
Molecule details ›
Chain: A
Length: 71 amino acids
Theoretical weight: 8.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43639 (Residues: 192-262; Coverage: 19%)
Gene names: GRB4, NCK2
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, molecular dynamics, rigid body/torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)