PDBe 1u54

X-ray diffraction
2.8Å resolution

Crystal Structures of the Phosphorylated and Unphosphorylated Kinase Domains of the CDC42-associated Tyrosine Kinase ACK1 bound to AMP-PCP

Released:

Function and Biology Details

Reactions catalysed:
ATP + a protein = ADP + a phosphoprotein. 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Activated CDC42 kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 33.14 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q07912 (Residues: 109-395; Coverage: 28%)
Gene names: ACK1, TNK2
Sequence domains: Protein tyrosine kinase
Structure domains:
Activated CDC42 kinase 1 Chain: B
Molecule details ›
Chain: B
Length: 291 amino acids
Theoretical weight: 33.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q07912 (Residues: 109-395; Coverage: 28%)
Gene names: ACK1, TNK2
Sequence domains: Protein tyrosine kinase
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2
Unit cell:
a: 200.384Å b: 42.332Å c: 70.848Å
α: 90° β: 96.28° γ: 90°
R-values:
R R work R free
0.226 0.222 0.322
Expression system: Spodoptera frugiperda