› 1u3h

X-ray diffraction
2.42Å resolution

Crystal structure of mouse TCR 172.10 complexed with MHC class II I-Au molecule at 2.4 A

Released:
DOI: 10.2210/pdb1u3h/pdb
PDB entry 1u3h coloured by chain, front view.
PDB entry 1u3h coloured by chain, side view.
PDB entry 1u3h coloured by chain, top view.
Source organisms:
Primary publication:
Structure of an autoimmune T cell receptor complexed with class II peptide-MHC: insights into MHC bias and antigen specificity.
Maynard J, Petersson K, Wilson DH, Adams EJ, Blondelle SE, Boulanger MJ, Wilson DB, Garcia KC
Immunity 22 81-92 (2005)
PMID: 15664161

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-137509 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Ig-like domain-containing protein Chains: A, E
Molecule details ›
Chains: A, E
Length: 110 amino acids
Theoretical weight: 12.29 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5R1B3 (Residues: 30-120; Coverage: 92%)
Gene name: TRAV14-3
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
T-cell receptor beta chain V region C5 Chains: B, F
Molecule details ›
Chains: B, F
Length: 111 amino acids
Theoretical weight: 12.11 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P04213 (Residues: 11-122; Coverage: 97%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
H-2 class II histocompatibility antigen, A-U alpha chain Chains: C, G
Molecule details ›
Chains: C, G
Length: 182 amino acids
Theoretical weight: 20.67 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P14438 (Residues: 1-179; Coverage: 79%)
Gene name: H2-Aa
Sequence domains:
Structure domains:
H-2 class II histocompatibility antigen, A-U beta chain Chains: D, H
Molecule details ›
Chains: D, H
Length: 189 amino acids
Theoretical weight: 22.5 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P06344 (Residues: 28-216; Coverage: 80%)
Sequence domains:
Structure domains:
Myelin basic protein Chains: I, P
Molecule details ›
Chains: I, P
Length: 12 amino acids
Theoretical weight: 1.3 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: P04370 (Residues: 135-142; Coverage: 3%)
Gene names: Mbp, Shi

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2221
Unit cell:
a: 87.835Å b: 327.162Å c: 127.161Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.274
Expression systems:
  • Escherichia coli BL21
  • Drosophila melanogaster
  • Not provided

Quick links

Citations

42 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
41 more

16 mentions without citation

Evolutionarily conserved amino acids that control TCR-MHC interaction.
Marrack et al. (2008)
15 more