PDBe 1u3f

X-ray diffraction
2.5Å resolution

Structural and Functional Characterization of a 5,10-Methenyltetrahydrofolate Synthetase from Mycoplasma pneumoniae (GI: 13508087)

Released:

Function and Biology Details

Reaction catalysed:
ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10- methenyltetrahydrofolate. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-formyltetrahydrofolate cyclo-ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 189 amino acids
Theoretical weight: 22.27 KDa
Source organism: Mycoplasma pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: P75430 (Residues: 1-164; Coverage: 100%)
Gene names: H91_orf164, MP488, MPN_348
Sequence domains: 5-formyltetrahydrofolate cyclo-ligase family
Structure domains: NagB/RpiA/CoA transferase-like

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 45.135Å b: 74.369Å c: 110.614Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.268
Expression system: Escherichia coli