PDBe 1u35

X-ray diffraction
3Å resolution

Crystal structure of the nucleosome core particle containing the histone domain of macroH2A

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (5 distinct):
Histone H3.1 Chains: A, E
Molecule details ›
Chains: A, E
Length: 136 amino acids
Theoretical weight: 15.44 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P68433 (Residues: 1-136; Coverage: 100%)
Gene names: H3.1-221, H3.1-291, H3.1-I, H3a, H3g, H3h, H3i, Hist1h3a, Hist1h3g, Hist1h3h, Hist1h3i
Sequence domains: Core histone H2A/H2B/H3/H4
Structure domains: Histone, subunit A
Histone H2B type 3-A Chains: D, H
Molecule details ›
Chains: D, H
Length: 126 amino acids
Theoretical weight: 14.03 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9D2U9 (Residues: 1-126; Coverage: 100%)
Gene name: Hist3h2ba
Sequence domains: Core histone H2A/H2B/H3/H4
Structure domains: Histone, subunit A
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 103 amino acids
Theoretical weight: 11.39 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P62806 (Residues: 1-103; Coverage: 100%)
Gene names: H4-12, H4-53, Hist1h4a, Hist1h4b, Hist1h4c, Hist1h4d, Hist1h4f, Hist1h4h, Hist1h4i, Hist1h4j, Hist1h4k, Hist1h4m, Hist2h4, Hist2h4a, Hist4h4
Sequence domains: Centromere kinetochore component CENP-T histone fold
Structure domains: Histone, subunit A
Core histone macro-H2A.1 Chains: C, G
Molecule details ›
Chains: C, G
Length: 120 amino acids
Theoretical weight: 12.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75367 (Residues: 1-120; Coverage: 32%)
Gene names: H2AFY, MACROH2A1
Sequence domains: Core histone H2A/H2B/H3/H4
Structure domains: Histone, subunit A
alpha-satellite DNA Chains: I, J
Molecule details ›
Chains: I, J
Length: 146 nucleotides
Theoretical weight: 45.05 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 105.505Å b: 109.598Å c: 175.989Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.26
Expression system: Escherichia coli