› 1tyb

X-ray diffraction
2.5Å resolution

STRUCTURAL ANALYSIS OF A SERIES OF MUTANTS OF TYROSYL-TRNA SYNTHETASE: ENHANCEMENT OF CATALYSIS BY HYDROPHOBIC INTERACTIONS

Released:
DOI: 10.2210/pdb1tyb/pdb
PDB entry 1tyb coloured by chain, front view.
PDB entry 1tyb coloured by chain, side view.
PDB entry 1tyb coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Entry authors: Brown KA, De Meester P, Blow DM

Function and Biology Details

Reaction catalysed: 
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133714 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine--tRNA ligase Chain: E
Molecule details ›
Chain: E
Length: 319 amino acids
Theoretical weight: 36.28 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
UniProt:
  • Canonical: P00952 (Residues: 1-319; Coverage: 76%)
Gene name: tyrS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TYR 1 x TYR
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 64.46Å b: 64.46Å c: 237.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 not available not available
Expression system: Not provided

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