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Structural and functional analysis of the gpsA gene product of Archaeoglobus fulgidus: a glycerol-3-phosphate dehydrogenase with an unusual NADP+ preference.

Sakasegawa S, Hagemeier CH, Thauer RK, Essen LO, Shima S
Protein Sci 13 3161-71 (2004)
Cited: 8 times
EuropePMC logo PMID: 15557260

Abstract

NAD(+)-dependent glycerol-3-phosphate dehydrogenase (G3PDH) is generally absent in archaea, because archaea, unlike eukaryotes and eubacteria, utilize glycerol-1-phosphate instead of glycerol-3-phosphate for the biosynthesis of membrane lipids. Surprisingly, the genome of the hyperthermophilic archaeon Archaeoglobus fulgidus comprises a G3PDH ortholog, gpsA, most likely due to horizontal gene transfer from a eubacterial organism. Biochemical characterization proved G3PDH-like activity of the recombinant gpsA gene product. However, unlike other G3PDHs, the up to 85 degrees C thermostable A. fulgidus G3PDH exerted a 15-fold preference for NADPH over NADH. The A. fulgidus G3PDH bears the hallmarks of adaptation to halotolerance and thermophilicity, because its 1.7-A crystal structure showed a high surface density for negative charges and 10 additional intramolecular salt bridges compared to a mesophilic G3PDH structure. Whereas all amino acid residues required for dihydroxyacetone phosphate binding and reductive catalysis are highly conserved, the binding site for the adenine moiety of the NAD(P) cosubstrate shows a structural variation that reflects the observed NADPH preference, for example, by a putative salt bridge between R49 and the 2'-phosphate.

Articles - 1txg mentioned but not cited (2)

  1. Structural and functional analysis of the gpsA gene product of Archaeoglobus fulgidus: a glycerol-3-phosphate dehydrogenase with an unusual NADP+ preference. Sakasegawa S, Hagemeier CH, Thauer RK, Essen LO, Shima S. Protein Sci 13 3161-3171 (2004)
  2. Structure of glycerol-3-phosphate dehydrogenase (GPD1) from Saccharomyces cerevisiae at 2.45 Å resolution. Alarcon DA, Nandi M, Carpena X, Fita I, Loewen PC. Acta Crystallogr Sect F Struct Biol Cryst Commun 68 1279-1283 (2012)


Reviews citing this publication (1)

  1. Glycerol metabolism of haloarchaea. Williams TJ, Allen M, Tschitschko B, Cavicchioli R. Environ Microbiol 19 864-877 (2017)

Articles citing this publication (5)

  1. Activity and transcriptional regulation of bacterial protein-like glycerol-3-phosphate dehydrogenase of the haloarchaea in Haloferax volcanii. Rawls KS, Martin JH, Maupin-Furlow JA. J Bacteriol 193 4469-4476 (2011)
  2. Enzyme-driven speciation: crystallizing Archaea via lipid capture. Payandeh J, Pai EF. J Mol Evol 64 364-374 (2007)
  3. Metabolic engineering strategies for optimizing acetate reduction, ethanol yield and osmotolerance in Saccharomyces cerevisiae. Papapetridis I, van Dijk M, van Maris AJA, Pronk JT. Biotechnol Biofuels 10 107 (2017)
  4. Birth of Archaeal Cells: Molecular Phylogenetic Analyses of G1P Dehydrogenase, G3P Dehydrogenases, and Glycerol Kinase Suggest Derived Features of Archaeal Membranes Having G1P Polar Lipids. Yokobori SI, Nakajima Y, Akanuma S, Yamagishi A. Archaea 2016 1802675 (2016)
  5. Sugar analog synthesis by in vitro biocatalytic cascade: A comparison of alternative enzyme complements for dihydroxyacetone phosphate production as a precursor to rare chiral sugar synthesis. Hartley CJ, French NG, Scoble JA, Williams CC, Churches QI, Frazer AR, Taylor MC, Coia G, Simpson G, Turner NJ, Scott C. PLoS One 12 e0184183 (2017)


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