PDBe 1tw3

X-ray diffraction
2.35Å resolution

Crystal structure of Carminomycin-4-O-methyltransferase (DnrK) in complex with S-adenosyl-L-homocystein (SAH) and 4-methoxy-e-rhodomycin T (M-ET)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carminomycin 4-O-methyltransferase DnrK Chains: A, B
Molecule details ›
Chains: A, B
Length: 360 amino acids
Theoretical weight: 39.14 KDa
Source organism: Streptomyces peucetius
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06528 (Residues: 2-356; Coverage: 100%)
Gene name: dnrK
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: C2
Unit cell:
a: 210.69Å b: 53.134Å c: 83.07Å
α: 90° β: 105.03° γ: 90°
R-values:
R R work R free
0.196 0.193 0.244
Expression system: Escherichia coli