PDBe 1tmq

X-ray diffraction
2.5Å resolution

STRUCTURE OF TENEBRIO MOLITOR LARVAL ALPHA-AMYLASE IN COMPLEX WITH RAGI BIFUNCTIONAL INHIBITOR

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alpha-amylase Chain: A
Molecule details ›
Chain: A
Length: 471 amino acids
Theoretical weight: 51.26 KDa
Source organism: Tenebrio molitor
UniProt:
  • Canonical: P56634 (Residues: 1-471; Coverage: 100%)
Sequence domains:
Structure domains:
Alpha-amylase/trypsin inhibitor Chain: B
Molecule details ›
Chain: B
Length: 117 amino acids
Theoretical weight: 12.72 KDa
Source organism: Eleusine coracana
UniProt:
  • Canonical: P01087 (Residues: 1-117; Coverage: 96%)
Sequence domains: Protease inhibitor/seed storage/LTP family
Structure domains: Plant lipid-transfer and hydrophobic proteins

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 79.16Å b: 186.87Å c: 111.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.261