PDBe 1tml

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF A THERMOPHILIC ENDOCELLULASE

Released:
Source organism: Thermobifida fusca
Primary publication:
Crystal structure of the catalytic domain of a thermophilic endocellulase.
Biochemistry 32 9906-16 (1993)
PMID: 8399160

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase E-2 Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 30.44 KDa
Source organism: Thermobifida fusca
Expression system: Not provided
UniProt:
  • Canonical: P26222 (Residues: 32-317; Coverage: 70%)
Gene name: celB
Sequence domains: Glycosyl hydrolases family 6
Structure domains: Glycosyl hydrolases family 6, cellulases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 43.35Å b: 65.94Å c: 43.41Å
α: 90° β: 107.69° γ: 90°
R-values:
R R work R free
0.184 0.184 not available
Expression system: Not provided