PDBe 1th1

X-ray diffraction
2.5Å resolution

Beta-catenin in complex with a phosphorylated APC 20aa repeat fragment

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Catenin beta-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 532 amino acids
Theoretical weight: 58.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35222 (Residues: 133-664; Coverage: 68%)
Gene names: CTNNB, CTNNB1, OK/SW-cl.35, PRO2286
Sequence domains: Armadillo/beta-catenin-like repeat
Structure domains: Leucine-rich Repeat Variant
Adenomatous polyposis coli protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 179 amino acids
Theoretical weight: 19.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P25054 (Residues: 1362-1540; Coverage: 6%)
Gene names: APC, DP2.5
Sequence domains: APC repeat

Ligands and Environments

No bound ligands

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 85.159Å b: 133.8Å c: 82.166Å
α: 90° β: 110.85° γ: 90°
R-values:
R R work R free
0.225 0.225 0.257
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli