PDBe 1tf6

X-ray diffraction
3.1Å resolution

CO-CRYSTAL STRUCTURE OF XENOPUS TFIIIA ZINC FINGER DOMAIN BOUND TO THE 5S RIBOSOMAL RNA GENE INTERNAL CONTROL REGION

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor IIIA Chains: A, D
Molecule details ›
Chains: A, D
Length: 190 amino acids
Theoretical weight: 22.13 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03001 (Residues: 23-212; Coverage: 52%)
Gene name: gtf3a
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
DNA (5'-D(*AP*CP*GP*GP*GP*CP*CP*TP*GP*GP*TP*TP*AP*GP*TP*AP*C P*CP*TP*GP*GP*AP* TP*GP*GP*GP*AP*GP*AP*CP*C)-3') Chains: B, E
Molecule details ›
Chains: B, E
Length: 31 nucleotides
Theoretical weight: 9.63 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*GP*GP*TP*CP*TP*CP*CP*CP*AP*TP*CP*CP*AP*GP*GP*T P*AP*CP*TP*AP*AP* CP*CP*AP*GP*GP*CP*CP*CP*G)-3') Chains: C, F
Molecule details ›
Chains: C, F
Length: 31 nucleotides
Theoretical weight: 9.43 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P1
Unit cell:
a: 64.18Å b: 64.712Å c: 78.035Å
α: 90.07° β: 92.98° γ: 102.95°
R-values:
R R work R free
0.308 0.308 0.363
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided