› 1t7e

X-ray diffraction
1.4Å resolution

Crystal structure of mutant Pro9Ser of scorpion alpha-like neurotoxin BmK M1 from Buthus martensii Karsch

Released:
DOI: 10.2210/pdb1t7e/pdb
PDB entry 1t7e coloured by chain, front view.
PDB entry 1t7e coloured by chain, side view.
PDB entry 1t7e coloured by chain, top view.
Source organism: Mesobuthus martensii
Primary publication:
Structural mechanism governing cis and trans isomeric states and an intramolecular switch for cis/trans isomerization of a non-proline peptide bond observed in crystal structures of scorpion toxins.
Guan RJ, Xiang Y, He XL, Wang CG, Wang M, Zhang Y, Sundberg EJ, Wang DC
J Mol Biol 341 1189-204 (2004)
PMID: 15321715

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155330 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-like toxin BmK M1 Chain: A
Molecule details ›
Chain: A
Length: 66 amino acids
Theoretical weight: 7.43 KDa
Source organism: Mesobuthus martensii
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P45697 (Residues: 20-83; Coverage: 99%)
Sequence domains: Scorpion toxin-like domain
Structure domains: Knottin, scorpion toxin-like

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P3221
Unit cell:
a: 46.907Å b: 46.907Å c: 53.748Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.192 0.216
Expression system: Saccharomyces cerevisiae

Quick links

Citations

3 review citations

Voltage-gated sodium channel modulation by scorpion alpha-toxins.
Bosmans et al. (2007)
2 more