PDBe 1t75

X-ray diffraction
2.5Å resolution

Crystal structure of Escherichia coli beta carbonic anhydrase

Released:
Source organism: Escherichia coli
Entry authors: Oganesyan V, Kim S-H, Kim R, Jancarik J, Berkeley Structural Genomics Center (BSGC)

Function and Biology Details

Reaction catalysed:
H(2)CO(3) = CO(2) + H(2)O. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 2 Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 220 amino acids
Theoretical weight: 25.13 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P61517 (Residues: 1-220; Coverage: 100%)
Gene names: JW0122, b0126, can, cynT2, yadF
Sequence domains: Carbonic anhydrase
Structure domains: Beta-carbonic Anhydrase; Chain A

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P43212
Unit cell:
a: 110.447Å b: 110.447Å c: 162.536Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.246
Expression system: Escherichia coli K-12