PDBe 1t5x

X-ray diffraction
2.5Å resolution

HLA-DR1 in complex with a synthetic peptide (AAYSDQATPLLLSPR) and the superantigen SEC3-3B2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Enterotoxin type C-3 Chain: D
Molecule details ›
Chain: D
Length: 239 amino acids
Theoretical weight: 27.72 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A0L5 (Residues: 28-266; Coverage: 100%)
Gene name: entC3
Sequence domains:
Structure domains:
Regulatory protein MIG1 Chain: C
Molecule details ›
Chain: C
Length: 15 amino acids
Theoretical weight: 1.6 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: P27705 (Residues: 455-462; Coverage: 2%)
Gene names: CAT4, MIG1, SSN1, YGL035C
HLA class II histocompatibility antigen, DR alpha chain Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 21.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01903 (Residues: 27-207; Coverage: 79%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DRB1-1 beta chain Chain: B
Molecule details ›
Chain: B
Length: 190 amino acids
Theoretical weight: 22.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04229 (Residues: 30-219; Coverage: 80%)
Gene name: HLA-DRB1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: R3
Unit cell:
a: 173.208Å b: 173.208Å c: 121.543Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.208 0.241
Expression systems:
  • Escherichia coli
  • Not provided
  • Escherichia coli BL21(DE3)