› 1t2x

X-ray diffraction
2.3Å resolution

Glactose oxidase C383S mutant identified by directed evolution

Released:
DOI: 10.2210/pdb1t2x/pdb
PDB entry 1t2x coloured by chain, front view.
PDB entry 1t2x coloured by chain, side view.
PDB entry 1t2x coloured by chain, top view.
Source organism: Fusarium sp.
Primary publication:
Structural and kinetic studies of a series of mutants of galactose oxidase identified by directed evolution.
Wilkinson D, Akumanyi N, Hurtado-Guerrero R, Dawkes H, Knowles PF, Phillips SE, McPherson MJ
Protein Eng Des Sel 17 141-8 (2004)
PMID: 15047910

Function and Biology Details

Reaction catalysed: 
D-galactose + O(2) = D-galacto-hexodialdose + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactose oxidase Chain: A
Molecule details ›
Chain: A
Length: 639 amino acids
Theoretical weight: 68.56 KDa
Source organism: Fusarium sp.
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P0CS93 (Residues: 42-680; Coverage: 97%)
Gene name: GAOA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand CU 1 x CU
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: C2
Unit cell:
a: 97.376Å b: 88.995Å c: 85.8Å
α: 90° β: 117.49° γ: 90°
R-values:
R R work R free
0.218 0.218 0.228
Expression system: Komagataella pastoris

Quick links

Citations

3 review citations

From protein engineering to immobilization: promising strategies for the upgrade of industrial enzymes.
Singh et al. (2013)
2 more

1 mention without citation

Copper active sites in biology.
Solomon et al. (2014)