PDBe 1t2s

Solution NMR

Structural basis for 3' end recognition of nucleic acids by the Drosophila Argonaute 2 PAZ domain

Released:
Primary publication:
Nucleic acid 3'-end recognition by the Argonaute2 PAZ domain.
Nat. Struct. Mol. Biol. 11 576-7 (2004)
PMID: 15156196

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Protein argonaute-2 Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.93 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9VUQ5 (Residues: 602-720; Coverage: 10%)
Gene names: AGO2, CG7439
Structure domains: paz domain
5'-D(*CP*TP*CP*AP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 5 nucleotides
Theoretical weight: 1.44 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: MOLECULAR DYNAMICS, SIMULATED ANNEALING
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided