PDBe 1t2k

X-ray diffraction
3Å resolution

Structure Of The DNA Binding Domains Of IRF3, ATF-2 and Jun Bound To DNA

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Transcription factor AP-1 Chain: C
Molecule details ›
Chain: C
Length: 62 amino acids
Theoretical weight: 7.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05412 (Residues: 254-314; Coverage: 18%)
Gene name: JUN
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Interferon regulatory factor 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 112 amino acids
Theoretical weight: 13.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14653 (Residues: 1-112; Coverage: 26%)
Gene name: IRF3
Sequence domains: Interferon regulatory factor transcription factor
Structure domains: "winged helix" repressor DNA binding domain
Cyclic AMP-dependent transcription factor ATF-2 Chain: D
Molecule details ›
Chain: D
Length: 61 amino acids
Theoretical weight: 7.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15336 (Residues: 354-414; Coverage: 12%)
Gene names: ATF2, CREB2, CREBP1
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
31-MER Chain: E
Molecule details ›
Chain: E
Length: 31 nucleotides
Theoretical weight: 9.72 KDa
31-MER Chain: F
Molecule details ›
Chain: F
Length: 31 nucleotides
Theoretical weight: 9.34 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: C2
Unit cell:
a: 186.38Å b: 65.21Å c: 83.85Å
α: 90° β: 93.44° γ: 90°
R-values:
R R work R free
0.257 0.257 0.296
Expression system: Escherichia coli BL21(DE3)