PDBe 1t0j

X-ray diffraction
2Å resolution

Crystal structure of a complex between voltage-gated calcium channel beta2a subunit and a peptide of the alpha1c subunit

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Voltage-dependent L-type calcium channel subunit beta-2 Chain: A
Molecule details ›
Chain: A
Length: 132 amino acids
Theoretical weight: 15 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VGC3 (Residues: 68-196; Coverage: 20%)
Gene names: Cacnb2, Cacnlb2
Sequence domains: Voltage gated calcium channel subunit beta domain 4Aa N terminal
Structure domains: SH3 Domains
Voltage-dependent L-type calcium channel subunit beta-2 Chain: B
Molecule details ›
Chain: B
Length: 224 amino acids
Theoretical weight: 25.32 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VGC3 (Residues: 254-476; Coverage: 34%)
Gene names: Cacnb2, Cacnlb2
Sequence domains: Guanylate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Voltage-dependent L-type calcium channel subunit alpha-1C Chain: C
Molecule details ›
Chain: C
Length: 20 amino acids
Theoretical weight: 2.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13936 (Residues: 428-445; Coverage: 1%)
Gene names: CACH2, CACN2, CACNA1C, CACNL1A1, CCHL1A1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 36.756Å b: 45.33Å c: 60.652Å
α: 96.81° β: 102.46° γ: 98.53°
R-values:
R R work R free
0.202 0.2 0.242
Expression system: Escherichia coli