Structure analysis

Structural basis for nicotinamide cleavage and ADP-ribose transfer by NAD+-dependent Sir2 histone/protein deacetylases

X-ray diffraction
1.5Å resolution
Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents: 2 distinct polypeptide molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero dimer
Accessible surface area: 13600 Å2
Buried surface area: 3100 Å2
Dissociation area: 700 Å2
Dissociation energy (ΔGdiss): 4 kcal/mol
Dissociation entropy (TΔSdiss): 7 kcal/mol
Interface energy (ΔGint): -22 kcal/mol
Symmetry number: 1
Assembly 2
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Multimeric state: hetero tetramer
Accessible surface area: 25000 Å2
Buried surface area: 8300 Å2
Dissociation area: 1,000 Å2
Dissociation energy (ΔGdiss): 7 kcal/mol
Dissociation entropy (TΔSdiss): 14 kcal/mol
Interface energy (ΔGint): -57 kcal/mol
Symmetry number: 2

Macromolecules

Chain: B
Length: 10 amino acids
Theoretical weight: 1.2 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: P02309 (Residues: 13-22; Coverage: 10%)
Gene names: HHF1, HHF2, N2752, YBR009C, YBR0122, YNL030W

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Chain: A
Length: 297 amino acids
Theoretical weight: 33.48 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P53686 (Residues: 1-294; Coverage: 82%)
Gene names: HST2, LPA2C, YPL015C
Pfam: Sir2 family
InterPro:
CATH:
SCOP: Sir2 family of transcriptional regulators

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