PDBe 1szd

X-ray diffraction
1.5Å resolution

Structural basis for nicotinamide cleavage and ADP-ribose transfer by NAD+-dependent Sir2 histone/protein deacetylases


Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone H4 Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.2 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
  • Canonical: P02309 (Residues: 13-22; Coverage: 10%)
Gene names: HHF1, HHF2, N2752, YBR009C, YBR0122, YNL030W
NAD-dependent protein deacetylase HST2 Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 33.48 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
  • Canonical: P53686 (Residues: 1-294; Coverage: 82%)
Gene names: HST2, LPA2C, YPL015C
Sequence domains: Sir2 family
Structure domains:

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3221
Unit cell:
a: 105.383Å b: 105.383Å c: 66.15Å
α: 90° β: 90° γ: 120°
R R work R free
0.225 0.214 0.23
Expression systems:
  • Not provided
  • Escherichia coli