› 1spi

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF SPINACH CHLOROPLAST FRUCTOSE-1,6-BISPHOSPHATASE AT 2.8 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1spi/pdb
PDB entry 1spi coloured by chain, front view.
PDB entry 1spi coloured by chain, side view.
PDB entry 1spi coloured by chain, top view.
Source organism: Spinacia oleracea
Primary publication:
Crystal structure of spinach chloroplast fructose-1,6-bisphosphatase at 2.8 A resolution.
Villeret V, Huang S, Zhang Y, Xue Y, Lipscomb WN
Biochemistry 34 4299-306 (1995)
PMID: 7703243

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-1,6-bisphosphatase, chloroplastic Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 358 amino acids
Theoretical weight: 39.18 KDa
Source organism: Spinacia oleracea
Expression system: Not provided
UniProt:
  • Canonical: P22418 (Residues: 58-415; Coverage: 86%)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 76.1Å b: 85.7Å c: 105.8Å
α: 90° β: 103.1° γ: 90°
R-values:
R R work R free
0.203 0.203 not available
Expression system: Not provided

Quick links

Citations

12 review citations

Redox regulation: a broadening horizon.
Buchanan et al. (2005)
11 more

2 mentions without citation

Prediction of protein targets of kinetin using in silico and in vitro methods: a case study on spinach seed germination mechanism.
Kumar et al. (2015)
1 more