PDBe 1sll

X-ray diffraction
2Å resolution

SIALIDASE L FROM LEECH MACROBDELLA DECORA

Released:

Function and Biology Details

Reaction catalysed:
Elimination of alpha-sialyl groups in N-acetylneuraminic acid glycosides, releasing 2,7-anhydro-alpha-N-acetylneuraminate. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anhydrosialidase Chain: A
Molecule details ›
Chain: A
Length: 679 amino acids
Theoretical weight: 74.57 KDa
Source organism: Macrobdella decora
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q27701 (Residues: 81-759; Coverage: 92%)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P1
Unit cell:
a: 46.364Å b: 69.275Å c: 72.469Å
α: 113.48° β: 95.41° γ: 107.31°
R-values:
R R work R free
0.167 0.167 0.224
Expression system: Escherichia coli BL21(DE3)