PDBe 1sk6

X-ray diffraction
3.2Å resolution

Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin, 3',5' cyclic AMP (cAMP), and pyrophosphate

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-2 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 148 amino acids
Theoretical weight: 16.72 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P0DP24 (Residues: 2-149; Coverage: 99%)
Gene names: CALM2, CAM2, CAMB
Structure domains: EF-hand
Calmodulin-sensitive adenylate cyclase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 510 amino acids
Theoretical weight: 58.81 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
UniProt:
  • Canonical: P40136 (Residues: 291-800; Coverage: 67%)
Gene names: BXA0141, GBAA_pXO1_0142, cya, pXO1-122
Sequence domains: Anthrax toxin LF subunit
Structure domains: Adenylylcyclase toxin superfamily

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I222
Unit cell:
a: 116.87Å b: 166.453Å c: 342.393Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.25 0.307
Expression system: Escherichia coli