PDBe 1sc9

X-ray diffraction
1.8Å resolution

Hydroxynitrile Lyase from Hevea brasiliensis in complex with the natural substrate acetone cyanohydrin

Released:

Function and Biology Details

Reaction catalysed:
(1) An aliphatic (S)-hydroxynitrile = cyanide + an aliphatic aldehyde or ketone. (2) An aromatic (S)-hydroxynitrile = cyanide + an aromatic aldehyde. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(S)-hydroxynitrile lyase Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 29.26 KDa
Source organism: Hevea brasiliensis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P52704 (Residues: 1-257; Coverage: 100%)
Gene name: HNL
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: C2221
Unit cell:
a: 47.393Å b: 106.455Å c: 127.988Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.167 0.189
Expression system: Komagataella pastoris