PDBe 1sbt

X-ray diffraction
2.5Å resolution

ATOMIC COORDINATES FOR SUBTILISIN BPN (OR NOVO)

Released:
Source organism: Bacillus amyloliquefaciens
Primary publication:
Atomic coordinates for subtilisin BPN' (or Novo).
Biochem. Biophys. Res. Commun. 45 337-44 (1971)
PMID: 5160720

Function and Biology Details

Reaction catalysed:
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Subtilisin BPN' Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 27.55 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Not provided
UniProt:
  • Canonical: P00782 (Residues: 108-382; Coverage: 78%)
Gene name: apr
Sequence domains: Subtilase family
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 66.7Å b: 54.4Å c: 62.9Å
α: 90° β: 91.9° γ: 90°
Expression system: Not provided