PDBe 1sa3

X-ray diffraction
1.95Å resolution

An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type-2 restriction enzyme MspI Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 29.88 KDa
Source organism: Moraxella sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P11405 (Residues: 1-262; Coverage: 100%)
Gene name: mspIR
Sequence domains: Restriction endonuclease MspI
5'-D(*CP*CP*CP*CP*CP*GP*GP*GP*GP*G)-3' Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C, null
Spacegroup: P21
Unit cell:
a: 50.193Å b: 131.601Å c: 59.318Å
α: 90° β: 109.69° γ: 90°
R-values:
R R work R free
0.225 0.223 0.252
Expression systems:
  • Escherichia coli
  • Not provided