PDBe 1s9k

X-ray diffraction
3.1Å resolution

Crystal Structure of Human NFAT1 and Fos-Jun on the IL-2 ARRE1 Site

Released:
Source organism: Homo sapiens
Entry authors: Wang D, Stroud JC, Chen L

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Transcription factor AP-1 Chain: E
Molecule details ›
Chain: E
Length: 52 amino acids
Theoretical weight: 6.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05412 (Residues: 257-308; Coverage: 16%)
Gene name: JUN
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Proto-oncogene c-Fos Chain: D
Molecule details ›
Chain: D
Length: 53 amino acids
Theoretical weight: 6.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01100 (Residues: 140-192; Coverage: 14%)
Gene names: FOS, G0S7
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Nuclear factor of activated T-cells, cytoplasmic 2 Chain: C
Molecule details ›
Chain: C
Length: 280 amino acids
Theoretical weight: 31.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13469 (Residues: 399-678; Coverage: 30%)
Gene names: NFAT1, NFATC2, NFATP
Sequence domains: Rel homology DNA-binding domain
Structure domains:
Human IL-2 ARRE1 Promoter Element, Plus Strand Chain: A
Molecule details ›
Chain: A
Length: 20 nucleotides
Theoretical weight: 6.21 KDa
Human IL-2 ARRE1 Promoter Element, Minus Strand Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.05 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 64.769Å b: 86.26Å c: 84.044Å
α: 90° β: 111.24° γ: 90°
R-values:
R R work R free
0.242 0.242 0.275
Expression system: Escherichia coli