› 1s7u

X-ray diffraction
2.2Å resolution

Crystal structures of the murine class I major histocompatibility complex H-2Db in complex with LCMV-derived gp33 index peptide and three of its escape variants

Released:
DOI: 10.2210/pdb1s7u/pdb
PDB entry 1s7u coloured by chain, front view.
PDB entry 1s7u coloured by chain, side view.
PDB entry 1s7u coloured by chain, top view.
Source organisms:
Primary publication:
Determination of structural principles underlying three different modes of lymphocytic choriomeningitis virus escape from CTL recognition.
Velloso LM, Michaëlsson J, Ljunggren HG, Schneider G, Achour A
J Immunol 172 5504-11 (2004)
PMID: 15100292

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-134985 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, D-B alpha chain Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 338 amino acids
Theoretical weight: 38.45 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01899 (Residues: 25-362; Coverage: 100%)
Gene name: H2-D1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Stable signal peptide Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 9 amino acids
Theoretical weight: 1.05 KDa
Source organism: Lymphocytic choriomeningitis virus (strain WE)
Expression system: Not provided
UniProt:
  • Canonical: P07399 (Residues: 33-40; Coverage: 2%)
Gene names: GP-C, GPC

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 92.389Å b: 123.343Å c: 99.392Å
α: 90° β: 103.17° γ: 90°
R-values:
R R work R free
0.216 0.213 0.262
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

1 review citation

Structural aspects of chemical modifications in the MHC-restricted immunopeptidome; Implications for immune recognition.
Sandalova et al. (2022)
24 other articles

5 mentions without citation

Successive crystal structure snapshots suggest the basis for MHC class I peptide loading and editing by tapasin.
Hafstrand et al. (2019)
4 more