PDBe 1s6l

Solution NMR

Solution structure of MerB, the Organomercurial Lyase involved in the bacterial mercury resistance system

Released:

Function and Biology Details

Reaction catalysed:
An alkylmercury + H(+) = an alkane + Hg(2+). 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkylmercury lyase Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 23.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P77072 (Residues: 1-212; Coverage: 100%)
Gene name: merB
Sequence domains:
Structure domains: "winged helix" repressor DNA binding domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 74%
Refinement method: Simulated annealing, with a combination of torsion angle dynamics and cartesian dynamics
Chemical shifts: BMR6047  
Expression system: Escherichia coli BL21(DE3)