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Structural basis for substrate selection by T7 RNA polymerase

Source organism: Escherichia phage T7
Biochemical function: DNA-directed 5'-3' RNA polymerase activity
Biological process: DNA-templated transcription
Cellular component: not assigned

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EC 2.7.7.6: DNA-directed RNA polymerase

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Systematic name:
Nucleoside-triphosphate:RNA nucleotidyltransferase (DNA-directed)
Alternative Name(s):
  • C RNA formation factors
  • C ribonucleic acid formation factors
  • DNA-dependent RNA nucleotidyltransferase
  • DNA-dependent RNA polymerase
  • DNA-dependent ribonucleate nucleotidyltransferase
  • Deoxyribonucleic acid-dependent ribonucleic acid polymerase
  • RNA nucleotidyltransferase
  • RNA nucleotidyltransferase (DNA-directed)
  • RNA polymerase
  • RNA polymerase I
  • RNA polymerase II
  • RNA polymerase III
  • RNA transcriptase
  • Ribonucleate nucleotidyltransferase
  • Ribonucleate polymerase
  • Ribonucleic acid nucleotidyltransferase
  • Ribonucleic acid polymerase
  • Ribonucleic acid transcriptase
  • Ribonucleic polymerase
  • Ribonucleic transcriptase
  • Transcriptase

GO terms

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Sequence families

Pfam Protein families (Pfam)
PF00940
Domain description: DNA-dependent RNA polymerase
Occurring in:
  1. T7 RNA polymerase
The deposited structure of PDB entry 1s0v contains 4 copies of Pfam domain PF00940 (DNA-dependent RNA polymerase) in T7 RNA polymerase. Showing 1 copy in chain M [auth A].

PF14700
Domain description: DNA-directed RNA polymerase N-terminal
Occurring in:
  1. T7 RNA polymerase
The deposited structure of PDB entry 1s0v contains 4 copies of Pfam domain PF14700 (DNA-directed RNA polymerase N-terminal) in T7 RNA polymerase. Showing 1 copy in chain M [auth A].

InterPro InterPro annotations
IPR002092
Domain description: DNA-directed RNA polymerase, phage-type
Occurring in:
  1. T7 RNA polymerase
IPR029262
Domain description: DNA-directed RNA polymerase, N-terminal
Occurring in:
  1. T7 RNA polymerase
IPR046950
Domain description: DNA-directed RNA polymerase, C-terminal domain, phage-type
Occurring in:
  1. T7 RNA polymerase
IPR024075
Domain description: DNA-directed RNA polymerase, helix hairpin domain superfamily
Occurring in:
  1. T7 RNA polymerase
IPR037159
Domain description: DNA-directed RNA polymerase, N-terminal domain superfamily
Occurring in:
  1. T7 RNA polymerase
IPR043502
Domain description: DNA/RNA polymerase superfamily
Occurring in:
  1. T7 RNA polymerase

Structure domains

CATH CATH domains
3.30.70.370
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: Alpha-Beta Plaits
Homology: Alpha-Beta Plaits
Occurring in:
  1. T7 RNA polymerase
The deposited structure of PDB entry 1s0v contains 4 copies of CATH domain 3.30.70.370 (Alpha-Beta Plaits) in T7 RNA polymerase. Showing 1 copy in chain M [auth A].
1.10.150.20
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: DNA polymerase; domain 1
Homology: 5' to 3' exonuclease, C-terminal subdomain
Occurring in:
  1. T7 RNA polymerase
The deposited structure of PDB entry 1s0v contains 4 copies of CATH domain 1.10.150.20 (DNA polymerase; domain 1) in T7 RNA polymerase. Showing 1 copy in chain M [auth A].
1.10.287.280
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Helix Hairpins
Homology: Helix Hairpins
Occurring in:
  1. T7 RNA polymerase
The deposited structure of PDB entry 1s0v contains 4 copies of CATH domain 1.10.287.280 (Helix Hairpins) in T7 RNA polymerase. Showing 1 copy in chain M [auth A].
1.10.287.260
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Helix Hairpins
Homology: Helix Hairpins
Occurring in:
  1. T7 RNA polymerase
The deposited structure of PDB entry 1s0v contains 4 copies of CATH domain 1.10.287.260 (Helix Hairpins) in T7 RNA polymerase. Showing 1 copy in chain M [auth A].
1.10.1320.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: T7 RNA polymerase; domain 1
Homology: DNA-directed RNA polymerase, N-terminal domain
Occurring in:
  1. T7 RNA polymerase
The deposited structure of PDB entry 1s0v contains 4 copies of CATH domain 1.10.1320.10 (T7 RNA polymerase; domain 1) in T7 RNA polymerase. Showing 1 copy in chain M [auth A].
SCOP SCOP annotation
56691
Class: Multi-domain proteins (alpha and beta)
Fold: DNA/RNA polymerases
Superfamily: DNA/RNA polymerases
Occurring in:
  1. T7 RNA polymerase
The deposited structure of PDB entry 1s0v contains 4 copies of SCOP domain 56691 (T7 RNA polymerase) in T7 RNA polymerase. Showing 1 copy in chain M [auth A].

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