PDBe 1s0v

X-ray diffraction
3.2Å resolution

Structural basis for substrate selection by T7 RNA polymerase

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
T7 RNA polymerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 883 amino acids
Theoretical weight: 98.98 KDa
Source organism: Enterobacteria phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00573 (Residues: 1-496, 498-883; Coverage: 100%)
Gene name: 1
Sequence domains:
Structure domains:
5'-D(*GP*TP*CP*GP*AP*TP*TP*CP*CP*C)-3' Chains: G, J, M, P
Molecule details ›
Chains: G, J, M, P
Length: 10 nucleotides
Theoretical weight: 3 KDa
5'-D(*G*GP*GP*AP*AP*TP*CP*GP*AP*TP*AP*TP*CP*GP*CP*CP*GP*C)-3' Chains: E, H, K, N
Molecule details ›
Chains: E, H, K, N
Length: 18 nucleotides
Theoretical weight: 5.54 KDa
5'-R(*AP*AP*CP*U*GP*CP*GP*GP*CP*GP*AP*U)-3' Chains: F, I, L, O
Molecule details ›
Chains: F, I, L, O
Length: 12 nucleotides
Theoretical weight: 3.85 KDa

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE
Spacegroup: P1
Unit cell:
a: 81.133Å b: 87.703Å c: 206.531Å
α: 91.93° β: 91.02° γ: 110.66°
R-values:
R R work R free
0.255 0.255 0.307
Expression system: Escherichia coli