PDBe 1rzz

X-ray diffraction
2.4Å resolution

PHOTOSYNTHETIC REACTION CENTER DOUBLE MUTANT FROM RHODOBACTER SPHAEROIDES WITH ASP L213 REPLACED WITH ASN AND ARG M233 REPLACED WITH CYS IN THE CHARGE-NEUTRAL DQAQB STATE (TETRAGONAL FORM)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein H chain Chains: H, T
Molecule details ›
Chains: H, T
Length: 260 amino acids
Theoretical weight: 28.07 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 1-260; Coverage: 100%)
Gene name: puhA
Sequence domains:
Structure domains:
Reaction center protein L chain Chains: L, R
Molecule details ›
Chains: L, R
Length: 281 amino acids
Theoretical weight: 31.35 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Membrane spanning alpha-helix pairs
Reaction center protein M chain Chains: M, S
Molecule details ›
Chains: M, S
Length: 307 amino acids
Theoretical weight: 34.34 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-308; Coverage: 100%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Membrane spanning alpha-helix pairs

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P43212
Unit cell:
a: 139.582Å b: 139.582Å c: 274.731Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.238
Expression system: Rhodobacter sphaeroides