PDBe 1ry1

Electron Microscopy
12Å resolution

Structure of the signal recognition particle interacting with the elongation-arrested ribosome

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 14-mer (preferred)
Entry contents:
6 distinct polypeptide molecules
8 distinct RNA molecules
Macromolecules (14 distinct):
Rhodopsin Chain: S
Molecule details ›
Chain: S
Length: 18 amino acids
Theoretical weight: 2.12 KDa
Source organism: Tursiops truncatus
Expression system: Not provided
UniProt:
  • Canonical: O62798 (Residues: 50-67; Coverage: 5%)
Gene name: RHO
Signal recognition particle protein Chain: U
Molecule details ›
Chain: U
Length: 296 amino acids
Theoretical weight: 32.47 KDa
Source organism: Thermus aquaticus
Expression system: Not provided
UniProt:
  • Canonical: O07347 (Residues: 1-296; Coverage: 69%)
Gene name: ffh
Sequence domains:
Signal recognition particle 54 kDa protein Chain: W
Molecule details ›
Chain: W
Length: 109 amino acids
Theoretical weight: 12.47 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P14576 (Residues: 326-434; Coverage: 22%)
Gene name: Srp54
Sequence domains: Signal peptide binding domain
Signal recognition particle 9 kDa protein Chain: C
Molecule details ›
Chain: C
Length: 85 amino acids
Theoretical weight: 10 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P49458 (Residues: 2-86; Coverage: 99%)
Gene name: SRP9
Sequence domains: Signal recognition particle 9 kDa protein (SRP9)
Signal recognition particle 14 kDa protein Chain: D
Molecule details ›
Chain: D
Length: 106 amino acids
Theoretical weight: 12.11 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P37108 (Residues: 2-107; Coverage: 78%)
Gene name: SRP14
Sequence domains: Signal recognition particle 14kD protein
Signal recognition particle 19 kDa protein Chain: B
Molecule details ›
Chain: B
Length: 108 amino acids
Theoretical weight: 12.56 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P09132 (Residues: 14-120; Coverage: 74%)
Gene name: SRP19
Sequence domains: SRP19 protein
RNA (31-MER) Chain: N
Molecule details ›
Chain: N
Length: 31 nucleotides
Theoretical weight: 9.81 KDa
fragment of 7S RNA Chain: M
Molecule details ›
Chain: M
Length: 27 nucleotides
Theoretical weight: 8.78 KDa
ALU DOMAIN (RNA FRAGMENTS) Chain: E
Molecule details ›
Chain: E
Length: 50 nucleotides
Theoretical weight: 16.28 KDa
ALU DOMAIN (SRP9, SRP14 + RNA) Chain: A
Molecule details ›
Chain: A
Length: 128 nucleotides
Theoretical weight: 41.57 KDa
RNA (5'-R(P*AP*UP*CP*GP*CP*GP*CP*CP*UP*GP*UP*G)-3') Chain: R
Molecule details ›
Chain: R
Length: 12 nucleotides
Theoretical weight: 3.8 KDa
RNA (5'-R(P*CP*GP*AP*UP*CP*GP*GP*GP*UP*GP*UP*C)-3') Chain: Q
Molecule details ›
Chain: Q
Length: 12 nucleotides
Theoretical weight: 3.84 KDa
RNA (5'-R(*CP*AP*AP*UP*AP*GP*CP*CP*AP*CP*UP*GP*CP*AP*CP*UP*CP*CP*AP*G)-3') Chain: P
Molecule details ›
Chain: P
Length: 20 nucleotides
Theoretical weight: 6.33 KDa
RNA (5'-R(P*GP*UP*UP*CP*UP*GP*GP*GP*CP*UP*GP*UP*AP*GP*UP*GP*CP*GP*CP*UP*AP*UP*GP*C)-3') Chain: O
Molecule details ›
Chain: O
Length: 24 nucleotides
Theoretical weight: 7.7 KDa

Ligands and Environments

No bound ligands

2 modified residues:

Experiments and Validation Details

Entry percentile scores
Resolution: 12Å
Relevant EMDB volumes: EMD-1063
Expression system: Not provided