› 1rxl

Solution NMR

Solution structure of the engineered protein Afae-dsc

Released:
DOI: 10.2210/pdb1rxl/pdb
PDB entry 1rxl coloured by chain, ensemble of 15 models, front view.
PDB entry 1rxl coloured by chain, ensemble of 15 models, side view.
PDB entry 1rxl coloured by chain, ensemble of 15 models, top view.
Source organism: Escherichia coli
Primary publication:
An atomic resolution model for assembly, architecture, and function of the Dr adhesins.
Anderson KL, Billington J, Pettigrew D, Cota E, Simpson P, Roversi P, Chen HA, Urvil P, du Merle L, Barlow PN, Medof ME, Smith RA, Nowicki B, Le Bouguénec C, Lea SM, Matthews S
Mol Cell 15 647-57 (2004)
PMID: 15327779

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Afimbrial adhesin AFA-III Chain: A
Molecule details ›
Chain: A
Length: 156 amino acids
Theoretical weight: 16.95 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57254 (Residues: 20-160; Coverage: 94%)
Gene names: afaE-3, afaE3
Sequence domains: Dr-family adhesin
Structure domains: Dr adhesin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 84%
Refinement method: ARIA-implemented distance geometry/simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

25 review citations

Bacterial adhesion and entry into host cells.
Pizarro-Cerdá et al. (2006)
24 more

4 mentions without citation

A subfamily of Dr adhesins of Escherichia coli bind independently to decay-accelerating factor and the N-domain of carcinoembryonic antigen.
Korotkova et al. (2006)
3 more