PDBe 1rvj

X-ray diffraction
2.75Å resolution

PHOTOSYNTHETIC REACTION CENTER DOUBLE MUTANT FROM RHODOBACTER SPHAEROIDES WITH ASP L213 REPLACED WITH ASN AND ARG H177 REPLACED WITH HIS

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 260 amino acids
Theoretical weight: 28.12 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 1-260; Coverage: 100%)
Gene name: puhA
Sequence domains:
Structure domains:
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 281 amino acids
Theoretical weight: 31.35 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Membrane spanning alpha-helix pairs
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 307 amino acids
Theoretical weight: 34.36 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-307; Coverage: 99%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Membrane spanning alpha-helix pairs

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P3121
Unit cell:
a: 138.242Å b: 138.242Å c: 184.396Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.218 0.237
Expression system: Rhodobacter sphaeroides