PDBe 1ru4

X-ray diffraction
1.6Å resolution

Crystal structure of pectate lyase Pel9A


Function and Biology Details

Reaction catalysed:
Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Pectate lyase L Chain: A
Molecule details ›
Chain: A
Length: 400 amino acids
Theoretical weight: 42.83 KDa
Source organism: Dickeya chrysanthemi
Expression system: Escherichia coli
  • Canonical: P0C1A7 (Residues: 26-425; Coverage: 100%)
Gene names: Dda3937_02794, pelL
Sequence domains: Right handed beta helix region
Structure domains: Single-stranded right-handed beta-helix, Pectin lyase-like

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P212121
Unit cell:
a: 55.33Å b: 57.83Å c: 114.93Å
α: 90° β: 90° γ: 90°
R R work R free
0.177 0.167 0.186
Expression system: Escherichia coli