› 1rtx

X-ray diffraction
1.8Å resolution

Crystal Structure of Synechocystis Hemoglobin with a Covalent Heme Linkage

Released:
DOI: 10.2210/pdb1rtx/pdb
PDB entry 1rtx coloured by chain, front view.
PDB entry 1rtx coloured by chain, side view.
PDB entry 1rtx coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
The crystal structure of Synechocystis hemoglobin with a covalent heme linkage.
Hoy JA, Kundu S, Trent JT, Ramaswamy S, Hargrove MS
J Biol Chem 279 16535-42 (2004)
PMID: 14736872

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Group 1 truncated hemoglobin GlbN Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.76 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P73925 (Residues: 2-124; Coverage: 99%)
Gene names: glbN, slr2097
Sequence domains: Bacterial-like globin
Structure domains: Globins

Ligands and Environments

4 bound ligands:
Ligand CD 2 x CD
Ligand SO4 1 x SO4
Ligand K 1 x K
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5, RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 42.5Å b: 46Å c: 61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.224
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Structure and reactivity of hexacoordinate hemoglobins.
Kakar et al. (2010)
4 more

7 mentions without citation

Structural characterization of ferric hemoglobins from three antarctic fish species of the suborder notothenioidei.
Vergara et al. (2007)
6 more