PDBe 1rtu

X-ray diffraction
1.8Å resolution

USTILAGO SPHAEROGENA RIBONUCLEASE U2

Released:

Function and Biology Details

Reaction catalysed:
Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in A-P or G-P with 2',3'-cyclic phosphate intermediates. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease U2 Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 12.39 KDa
Source organism: Ustilago sphaerogena
UniProt:
  • Canonical: P00654 (Residues: 1-114; Coverage: 100%)
Gene name: RNU2
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE
Spacegroup: P212121
Unit cell:
a: 49.32Å b: 61.27Å c: 34.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 not available not available