PDBe 1rtf

X-ray diffraction
2.3Å resolution

COMPLEX OF BENZAMIDINE WITH THE CATALYTIC DOMAIN OF HUMAN TWO CHAIN TISSUE PLASMINOGEN ACTIVATOR [(TC)-T-PA]

Released:

Function and Biology Details

Reaction catalysed:
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
TWO CHAIN TISSUE PLASMINOGEN ACTIVATOR Chain: A
Molecule details ›
Chain: A
Length: 17 amino acids
Theoretical weight: 2.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Tissue-type plasminogen activator chain B Chain: B
Molecule details ›
Chain: B
Length: 252 amino acids
Theoretical weight: 28.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00750 (Residues: 311-562; Coverage: 47%)
Gene name: PLAT
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 59.2Å b: 59.2Å c: 136.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.184 0.265
Expression system: Escherichia coli