PDBe 1rrp

X-ray diffraction
2.96Å resolution

STRUCTURE OF THE RAN-GPPNHP-RANBD1 COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero octamer
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTP-binding nuclear protein Ran Chains: A, C
Molecule details ›
Chains: A, C
Length: 204 amino acids
Theoretical weight: 23.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P62826 (Residues: 8-211; Coverage: 94%)
Gene names: ARA24, OK/SW-cl.81, RAN
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
E3 SUMO-protein ligase RanBP2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 134 amino acids
Theoretical weight: 15.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P49792 (Residues: 1171-1304; Coverage: 4%)
Gene names: NUP358, RANBP2
Sequence domains: RanBP1 domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: C2
Unit cell:
a: 121.339Å b: 139.463Å c: 91.654Å
α: 90° β: 135.78° γ: 90°
R-values:
R R work R free
0.252 0.252 0.304
Expression system: Escherichia coli BL21