› 1rmy

X-ray diffraction
1.75Å resolution

Crystal structure of AphA class B acid phosphatase/phosphotransferase ternary complex with deoxycytosine and phosphate bound to the catalytic metal

Released:
DOI: 10.2210/pdb1rmy/pdb
PDB entry 1rmy coloured by chain, front view.
PDB entry 1rmy coloured by chain, side view.
PDB entry 1rmy coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Calderone V, Forleo C, Benvenuti M, Rossolini GM, Thaller MC, Mangani S

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142401 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Class B acid phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 23.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE22 (Residues: 26-237; Coverage: 100%)
Gene names: JW4015, aphA, b4055, napA, yjbP
Sequence domains: HAD superfamily, subfamily IIIB (Acid phosphatase)
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand DCZ 2 x DCZ
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: C2
Unit cell:
a: 91.882Å b: 66.393Å c: 90.018Å
α: 90° β: 119.66° γ: 90°
R-values:
R R work R free
0.156 0.153 0.194
Expression system: Escherichia coli

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