PDBe 1rh9

X-ray diffraction
1.5Å resolution

Family GH5 endo-beta-mannanase from Lycopersicon esculentum (tomato)

Released:

Function and Biology Details

Reaction catalysed:
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans. 
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannan endo-1,4-beta-mannosidase 4 Chain: A
Molecule details ›
Chain: A
Length: 373 amino acids
Theoretical weight: 42.44 KDa
Source organism: Solanum lycopersicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8L5J1 (Residues: 27-399; Coverage: 100%)
Gene name: MAN4
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 59.54Å b: 74.521Å c: 79.006Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.205
Expression system: Escherichia coli