› 1rh6

X-ray diffraction
1.7Å resolution

Bacteriophage Lambda Excisionase (Xis)-DNA Complex

Released:
DOI: 10.2210/pdb1rh6/pdb
PDB entry 1rh6 coloured by chain, front view.
PDB entry 1rh6 coloured by chain, side view.
PDB entry 1rh6 coloured by chain, top view.
Source organism: Lambdavirus lambda
Primary publication:
Crystal structure of the excisionase-DNA complex from bacteriophage lambda.
Sam MD, Cascio D, Johnson RC, Clubb RT
J Mol Biol 338 229-40 (2004)
PMID: 15066428

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-114522 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Excisionase Chains: A, B
Molecule details ›
Chains: A, B
Length: 55 amino acids
Theoretical weight: 6.79 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03699 (Residues: 1-55; Coverage: 76%)
Gene name: xis
Sequence domains: Excisionase-like protein
Structure domains: Multidrug-efflux Transporter Regulator; Chain: A; Domain 2
5'-D(*CP*TP*AP*TP*GP*TP*AP*GP*TP*CP*TP*GP*TP*TP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.61 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided
5'-D(P*CP*AP*AP*CP*AP*GP*AP*CP*TP*AP*CP*AP*TP*AP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.57 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: C2
Unit cell:
a: 80.215Å b: 72.691Å c: 38.801Å
α: 90° β: 104.11° γ: 90°
R-values:
R R work R free
0.193 0.191 0.226
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

The many faces of the helix-turn-helix domain: transcription regulation and beyond.
Aravind et al. (2005)
2 more

8 mentions without citation

DNA conformations and their sequence preferences.
Svozil et al. (2008)
7 more